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KMID : 1007520090180040978
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Food Science and Biotechnology
2009 Volume.18 No. 4 p.978 ~ p.984
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Multiresidue Determination of Quinolones in Porcine, Chicken, and Bovine Muscle Using Liquid Chromatography with Fluorescence Detection
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Lee Sang-Hee
Shim You-Sin
Kim Hyun-Ju
Shin Dong-Bin
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Abstract
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An analytical method for the simultaneous determination of 9 quinolones (QNs) in porcine, chicken, and bovinemuscles was developed and validated using liquid chromatography-fluorescence detector (LC-FLD). The samples wereextracted using a liquid-liquid extraction (LLE) process. Chromatographic separation was achieved on a reverse phase C8column with a gradient elution using a mobile phase of 200 mM ammonium acetate buffer (pH 4.5) and acetonitrile (ACN).The proposed method was validated according to the Food and Drug Administration (FDA) guideline for bioanalytical assayprocedures. Recoveries of QNs were 83.1-111.9% with relative standard deviations (RSDs) below 15%. Linearity within arange of 30-500μg/kg was obtained with the correlation coefficient (R2) of 0.9967-0.9999. The limits of detection (LOD)were 1-16μg/kg. These values were lower than the maximum residues limits (MRLs) established by the European Union(EU). The present method was successfully applied to determine QNs in edible muscles.
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KEYWORD
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quinolone, bovine muscle, chicken muscle, porcine muscle, high pressure liquid chromatography-fluorescencedetector (HPLC-FLD), validation
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